Consistent Errors in First Strand cDNA Due to Random Hexamer Mispriming
نویسندگان
چکیده
منابع مشابه
Consistent Errors in First Strand cDNA Due to Random Hexamer Mispriming
Priming of random hexamers in cDNA synthesis is known to show sequence bias, but in addition it has been suggested recently that mismatches in random hexamer priming could be a cause of mismatches between the original RNA fragment and observed sequence reads. To explore random hexamer mispriming as a potential source of these errors, we analyzed two independently generated RNA-seq datasets of s...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0085583